摘要
本文应用PAP免疫组织化学技术对109例肝病患者肝组织中HBxAg的表达进行了检测。31例慢性活动性肝炎有29例阳性,其中22例(占75.9％)胞浆和胞核均阳性;6例只分布在胞浆中,一例仅在胞核中。40例肝炎后肝硬化及38例肝癌组全部阳性,均在胞浆中表达,仅有少数病例同时在胞核中显示(分别占12.5％和13.2％)。表明X蛋白既可存在于胞核中又可存在于胞浆中,以胞浆表达更为普遍,而在核中的定位与肝细胞的变性有关。HBxAg对免疫组化敏感性很强,较HBV其他抗原易检测,可作为检测HBV感染的标志之一。
The expression of HBxAg in liver tissues of 109 patients with chronic liver disease was observed by PAP immunohistochemistry technique. 29/31(93. 5%) patients with chronic active hepatitis was HBxAg positive expression in liver cells, in which, 22 cases(75%) were positive staining in both of the cyto-plasmand nuclei, another-6 cases were found only in the cytoplasm and one only in the nuclei. All of the 40 patients with cirrhosis and 38 patients with hepatocellular cancer were HBxAg positive exprission in the cytoplasm. Only a few cases (12. 5% and 13. 2%) were positive in both of nuclei and cytoplasm. It was indicated that the X protein presented not only in nuclei, but also in hepatocellular cytoplasm, which was more common. The local distribution of positive staining in the nuclei was related to the degeneration of liver cells. Detecting of HBxAg was more sensitive than that of HBsAg and HBcAg by immunohistochemistry. It is possible that the HBsAg expression in liver cells could be an additional new diagnostic marker for hepatitic B virus infection.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1993年第2期112-115,共4页
Immunological Journal
关键词
乙型肝炎病毒
X蛋白
肝炎
肝硬变
Hepatitis B virus. X protein, Chronic active hepatitis. Cirrhosis. Hepatocellular carcinoma
