摘要
目的 观察黄芪甲甙对大鼠肾缺血再灌注损伤 (ischemiareperfusioninjury ,IRI)远期单核细胞趋化蛋白 1(monocytechemoattractantprotein 1,MCP 1)的作用。 方法 采用Sprague Dawley大鼠右侧肾切除 ,左侧肾动脉夹闭 6 0min肾缺血后再灌注模型。实验动物分为肾缺血再灌注损伤组 (IRI) ,实验组 (AstragalosideIV ,Astr)和假手术组 (Sham) ,观察肾IRI大鼠术后 4、12和 2 4周血清肌酐 (Cr)、尿蛋白、肾组织的病理、胶原染色及MCP 1的变化。结果 IRI组尿总蛋白随时间延长进行性增加 ,皮髓质交界处胶原染色增强且在术后 2 4周肾间质中胶原增加 ,同时的MCP 1蛋白和mRNA表达增加。术后 12和 2 4周 ,Astr组上述改变较IRI组明显减轻 (P <0 .0 5 ) ,虽然略高于Sham组但无明显差别 (P >0 .0 5 )。结论 黄芪甲甙通过下调MCP
Purpose To elucidate long-term effect of astrgaloside Ⅳ on monocyte chemoattractant protein-1(MCP?1) in renal ischemia-reperfusion injury(IRI) in the rat. Methods Fifty-four male Sprague-Dawley rats (200～220 g) were randomized into IRI group,astrgaloside Ⅳ(Astr) group and Sham operation group(as control).10%(g/L) astrgaloside Ⅳ 2mL was given by daily gavage in Astr groups during operation for 6 days,other groups treated with equal dose of normal saline.After right kidney was removed,the renal artery of the left kidney were occluded with a vascular clamp for 60 minutes.Animals were killed at 4,12 and 24 weeks and kidney were havested for expression of MCP?1 mRNA and protein determination,collagen staining and monitoring proteinuria ,serum creatinine (Cr) were also performed. Results Animals in IRI group developed progressive proteinuria from 4 weeks onwards,expression of MCP?1 and scores of collagen staining were also significantly increased.At 12 and 24 weeks after operation,changes in Astr group above were significantly decreased than that of animals in IRI group(P<0.05),though higher than that of Sham group(P>05). Conclusions Astrgaloside Ⅳ markedly ameliorated renal injury by downregulation of MCP?1 expression.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2004年第6期588-591,i011,共5页
Fudan University Journal of Medical Sciences
基金
上海市科委重大项目基金资助 ( 0 2 41190 0 1)
