摘要
Objective:To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods: MC was cultured in vitro with different concentrations of glucose (30, 50, 80 mmol/L). The cell growth was observed by using MTT method and apoptosis by using an aunexin-V-Fluos. Immunohistochemical studies for Laminin (LN), Fibronectin (FN) and type Ⅳ Collagens (Col Ⅳ) were measured. Results: Shikonin inhibited their growth (P<0.05) and apoptosis in the glycated cultured cells. Shikonin 0.05 mmol/L significantly reduced the secretion of LN, FN and Col Ⅳ from MC (P<0.05) cultured in 30, 50 and 80 mmol/L glucose. Conclusion: Shikonin could prevent or treat diabetic nephropathy (DN) and glomerulosclerosis (GS).
目的 :研究紫草素对人胎肾小球系膜细胞(MC)增殖、凋亡及细胞外基质的影响。方法 :采用人胎肾细胞培养法、MTT法、流式细胞仪及免疫组化的方法。结果 :紫草素 0 .0 5、0 .10、0 .5 0及 1.0 0mmol L浓度对培养 2 4、4 8、72h人MC有明显抑制作用 ;紫草素明显抑制高糖诱发的人MC细胞凋亡 ;紫草素 0 .0 5、0 .10、0 .5 0 μg ml显著抑制系膜基质层粘连蛋白 (LN)、纤维连接蛋白 (FN)及胶原Ⅳ (CoⅣ )的生成。结论 :紫草素具抑制人MC增殖、细胞外基质生成及细胞凋亡作用。
基金
SupportedbytheGrantofJiangsuprovincepost Ph .D .ScientificResearchGrant
