摘要
Objective: To improve the method of pancreaticoduodenal transplantation and to establish a more physiological rat model. Methods: SD rats served as donors and recipients. The vein was reconstructed by end-to-side anastomosis between the donor portal vein and the recipient superior mesenteric vein, and arterial reconstruction was carried out by end-to-side anastomosis of the donor to the recipient abdominal aorta. Enteric drainage was performed by side-to-side anastomosis between the duodenum of donors and that of recipients. Results: Fifty experiments were performed. The successful rate of transplantation which restored the recipients euglycemia were 78%. Conclusion: This model of pancreaticoduodenal transplantation in rats was stable and reliable, which was in accordance with the trend of clinical pancreas transplantation and could be applied for further scientific research.
目的 :改进现有的胰十二指肠移植模型的建立方法 ,建立一种符合生理模式的大鼠胰十二指肠移植模型。方法 :运用显微外科技术 ,对链脲佐菌素诱导的糖尿病大鼠进行胰十二指肠移植。供胰的静脉回流采用符合生理的门静脉系统回流途径 ,即供胰的门静脉与受体肠系膜上静脉行端侧吻合。供胰的腹主动脉与受体腹主动脉行端侧吻合。供体十二指肠节断与受体十二指肠行侧侧吻合。结果 :共进行 5 0次大鼠胰十二指肠移植手术 ,术后血糖正常且存活超过 5天者共 39例 ,手术成功率为 78%。结论 :该模型稳定、可靠 ,符合生理模式 ,可用于进一步实验研究。
基金
SupportedbyaGrantfromthe"13 5"FoundationofJiangsuProvince
