摘要
目的 :研究乙型肝炎病毒标志物检测结果与 HBV- DNA定量检测的关系及临床意义。方法 :采用 Am plisensor定量 PCR系统测定 HBV- DNA含量 ,并用 EL ISA方法测定 HBV标志物 HBs Ag、HBe Ag。结果 :10 8例血清标本中 ,HBs Ag(+) HBe Ag(+)组的 HBV- DNA平均拷贝数为 10 7.52± 1 .6 5;HBs Ag(+) HBe Ag(- )组的 HBV- DNA平均拷贝数为 10 4 .6 1± 1 .1 4;HBs Ag (- ) HBe Ag (- )组的 HBV- DNA平均拷贝数为 10 3.0 7± 1 .0 2 ;正常对照组平均拷贝数为10 3.2 2± 1 .38。 HBs Ag(+) HBe Ag(+)组的 HBV- DNA平均拷贝数显著高于其它各组。结论 :HBV标志物检测结果与HBV- DNA定量结果相互印证 ,EL ISA法是一项灵敏度高、成本低、适用范围广的检测方法。
Objective: To research the relationship between HBV serum markers and HBV-DNA and its clinical significance. Methods: 108 serum samples were detected.The quantitative determination of HBV-DNA was by Amplisensor PCR. HBsAg and HBeAg were by enzyme linked immunosorbent assay(ELISA). Results: Average of HBV-DNA was 10 7.52±1.65 in group HBsAg(+) HBeAg(+). Average of HBV-DNA was 10 4.61±1.14 in group HBsAg(+) HBeAg(-). Average of HBV-DNA was 10 3.07±1.02 in group HBsAg(-) HBeAg(-). Average of HBV-DNA was 10 3.22±1.38 in healthy group. The HBsAg(+) HBeAg(+) group was obviously higher than the others. Conclusion: The results of HBV markers and HBV-DNA are verified each other. ELISA is a high sensitive method with low costs and wide-ranging application.
出处
《华夏医学》
2004年第6期884-885,共2页
Acta Medicinae Sinica
