摘要
目的 观察中药对体外培养新生小牛及先天性视网膜变性小鼠rds小鼠视网膜细胞生长的干预作用。方法 采用MTT法观察单味中药、复方、中药单体成分对新生小牛和rds小鼠体外培养视网膜神经细胞增殖率的影响。结果 复方 1、枸杞、枸杞多糖、当归、阿魏酸对牛视网膜神经细胞有明显的促增殖作用 ,黄芪、黄芪多糖、bFGF的保护作用较为温和 ,丹参无明显促增殖作用。各药物对rds小鼠的作用趋势与新生小牛视网膜细胞基本相同 ,复方 1对 2个种系的视网膜细胞均作用最强 ,浓度 5 g·L-1时新生小牛视网膜的增殖率为 16 2 % ,浓度 10g·L-1时rds小鼠视网膜的增殖率为 188.0 2 % ,bFGF对rds小鼠视网膜神经细胞无明显作用。结论 复方 1等中药对体外培养视网膜神经细胞生长有较好的保护作用。
Objective To study the effects of herbs-interv ention on the cultured retinal cells from neonatal calf and retinal degeneratio n slow(rds)mice.M ethods The cultured retinal cells of rds mice and neonatal calf were tr eated with formula,single herb and effective composition.The mitochondrial met abolic ability of the cells were examined by MTT. Results It had been found from MTT assay that formula 1,lycium bararum,lycium barbarum polsaccharide,angelica sinensis,ferulic acid had marked accelerate effect on the retinal cells proliferation of neonatal calf.Astragalus membranaceus,astr agalus membranaceus polsaccharide,bFGF had shown moderate acceleration of prol iferation.Salvia miltiorrhiza had no obvious effect of acceleration and anti-pr oliferation.The effects trend of these Chinese medicine were almost the same as on retinal cells of rds mice.Among them,formula 1 was the most effective agents, the retinal cells growth rate of neonatal calf was 162% treated by 5g·L -1 formula 1,188.02% of rds mice treated by 10g·L -1 formula 1,bFGF had no o bvious effect of acceleration on retinal cell of rds mice. Conclusion These results suggest that som e Chinese medicine like formula 1 have promising effect on the retinal cells.
出处
《眼科新进展》
CAS
2004年第6期422-425,共4页
Recent Advances in Ophthalmology
基金
国家自然科学基金资助 (编号 :30 1 71 1 73)~~
关键词
中药
视网膜细胞
干预作用
RDS小鼠
细胞培养
Chinese medicinal herbs
retinal cell
interve ntion effect
rds mice
cell culture
