摘要
为提高牛体外受精(BIVF)过程中屠宰牛卵巢的利用率,本研究将采集的110个牛卵巢先用常规的注射器抽吸法采卵后,再用刀片切割法采集了位于深层的卵泡卵母细胞,并对两种方法回收的卵子分别进行了BIVF实验。结果表明,在先用抽吸法采卵每头牛可得到9.88±2.42枚可用卵子的基础上,切割法采卵又可获得平均8.74±3.48枚可用卵子。这使得平均采卵数达到了每头牛18.62±2.59枚。体外受精后,由切割法回收的深层卵子其卵裂率和桑椹胚、囊胚发育率分别为43.01％(160/372),14.24％(53/372)和10.48％(39/372),均明显低于抽吸法回收卵的73.62％(201/273)、31.14％(85/273)和19.05％(52/273),(P<0.01)。本研究证明了抽吸后再切割的两次采卵法对提高牛卵巢采卵效率有着明显的效果,而且这部分深层卵泡卵母细胞用于体外受精后可发育为正常的早期胚胎,但各项发育指标尚有待于进一步提高。
In order to raise the productive efficiency of BIVF (bovine in vitro fertilization) embryos, 1 006 oocytes were reoverd from 110 ovaries of Mongolia cow by cutting ovaries after aspiration of the oocytes from their surface follicles and treated with BIVF. The results showed that the recov- ery rate of rank A oocytes could be increased from 9.88±2.42 to 18. 62±2.59 per cow by using the additional cutting method. After BIVF, the proportions of cleaved ova, morulae and blasto- cysts were 43.01% (160/372), 14.24% (53/327) and 10. 48% (39/372), respectively. They were lower than that of the oocytes recovered from aspiration method significantly, (P<0. 01). The re- sults suggested that (1) the oocytes collected from the deep folicles of the bovine overies by cut- ting method had a comparatively large quantitive potential and (2) these oocytes could be in vitro inseminated and cultivated to the normal transferable embryos, but the development rates still need to be improved in the future studies.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
1993年第5期528-531,共4页
Journal of Inner Mongolia University：Natural Science Edition
关键词
切割采卵法
体外受精
卵母细胞
牛
cutting method
deep folicle oocytes
recovery rate
development rate
