摘要
根据作者已测得的Contracaecumogmorhini复合种 (包括Contracaecumogmorhini和Contracaecummargolisi)线粒体DNAcox1基因的部分核苷酸序列 ,设计、合成了一对针对C .margolisi的特异性引物(CHL1,CHL2 ) ,通过PCR条件优化和扩增 ,C .margolisi扩增出 1条清晰的 30 6bp大小的特异DNA片段 ,而其他虫体如C .ogmorhini、拟地新线虫、鲁道夫对盲囊线虫、猪蛔虫、犬弓首蛔虫、猪有齿食道口线虫等 14个对照样本均未扩增出特异性片段。敏感性实验可检测到C .margolisiDNA的最低浓度为 0 34ng μL ,从而初步建立了鉴定C .ogmorhini复合种的特异PCR方法。该方法特异性强、敏感度高、重复性好 ,不仅可用于C .ogmorhini复合种的分类鉴定 ,也可用于它们所导致的寄生虫病的诊断、防制和流行病学调查 ,同时也为C .
Based on the mtDNA cox1 sequences of Contracaecum ogmorhini complex from different otariid hosts and geographical origins,we synthersiged a pair of species-specific primers(CHL1,CHL2)for Contracaecum margolisi.Under optimal conditions,a specific pcox1 fragment with 306bp in length was amplified by PCR while there was no cross-reaction with C.ogmorhini from the southern hemisphere or other parasites such as Pseudoterranova decipiens,Contracaecum rudolphill,Ascarid suum and so on.The DNA concentration of C.margolisi that could be detected was as low as 0 34ng/μl,which guarantee the specificity of this assay to be used as a species identification of C.ogmorhini complex,as an earlier diagnosis and epidemilogy survey for C.ogmorhini and C.margolisi infection,and as an important evidence and means for studying the distribution and further classification of C.ogmorhini complex in China.
出处
《寄生虫与医学昆虫学报》
CAS
2004年第4期207-213,共7页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家杰出青年科学基金项目 (No . 3 0 2 2 5 0 3 3 )
教育部留学回国人员科研启动基金项目
