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类固醇激素对牛输卵管上皮细胞特异性糖蛋白基因表达作用的研究 被引量：2

Effects of Different Steroid Hormones on Expression of Bovine Oviductal Epithelial Specific Glycoprotein Genes in Cultural Bovine Oviductal Epithelial Cells
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摘要 模拟发情期母牛体内类固醇激素水平,在培养乏情期母牛输卵管上皮细胞的体系中添加雌二醇(E2)、孕酮(P)和血小板激活因子(plateletactivatingfactor,PAF),以诱导细胞的分泌作用。借助Northern的斑点杂交技术分析输卵管上皮特异性糖蛋白(OGP)基因的mRNA水平,研究激素及生长因子对体外培养的乏情期母牛输卵管上皮细胞分泌OGP的影响。结果显示:E2、P和PAF可以促进乏情期母牛输卵管上皮细胞OGP基因的表达。 We have evaluated effects of 17β-estradiol (E_2), progesterone (P), platelet activating factor (PAF), E_2and PAF, P and PAF on expression of bovine oviductal epithelial OGP genes in cultural bovine ampullary oviductal epithelial cells from nonestrus with Northern dot-blotting. The result of dot-blotting showed that OGP mRNA existed in the cultural oviductal epithelial cells induced by E_2, P and PAF.
作者 苗向阳 方昌阁 杨文胜
机构地区 中国农业科学院畜牧研究所 中国农业大学农业生物技术国家重点实验室
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2004年第6期608-611,共4页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 中国农业科学院院长基金(9725-16)
关键词 上皮细胞 输卵管 类固醇激素 PAF 特异性 糖蛋白基因 斑点杂交技术 母牛 乏情 发情期 specific oviductal epithelial glycoprotein 17β-estrodial progesterone platelet activating factor mRNA
分类号 S858 [农业科学—临床兽医学] S823 [农业科学—畜牧学]
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参考文献17

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畜牧兽医学报
2004年 第6期

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