摘要
从卵巢采集的卵丘-卵母细胞复合体(COCs)经体外成熟培养24h后,随机分为2组分别用于孤雌激活与体外受精。在相同培养条件下,比较孤雌生殖胚与体外受精胚的发育率和发育速度。结果表明:将牛孤雌生殖胚与体外受精胚分别置于mSOFaa和mBECMaa培养液中培养,卵裂率(89.3%vs.80.1%;83.2%vs.82.5%)、囊胚发育率(27.5%vs.24.0%;28.9%vs.21.9%)和孵化率(58.7%vs.56.7%;51.5%vs.53.3%),均无显著差异(P>0.05);孤雌生殖胚与体外受精胚在体外发育的速度基本相同。对孤雌生殖胚胎进行培养可以代替体外受精胚胎筛选最佳体外培养系统。
Cumulus oocyte complexes(COCs)were aspirated from follicles (2～8 mm in diameter) of bovine ovaries, then matured for about 24 h. Matured oocytes were randomly allocated into two groups. Oocytes in Group 1 were fertilized. Oocytes in Group 2 were activated sequential treatment with ionomycin and 6-DMAP. The development rate of fertilized embryos was compared with that of parthenogenetic embryos cultured in mSOFaa or mBECMaa culture medium under the same culture condition. It indicated that there were no difference on cleavage percent (80.1% vs. 89.3%; 82.5% vs. 83.2%, respectively), blastocyte percent(24.0% vs. 27.5%; 21.9% vs. 28.9%, respectively) or hatching percent (56.7% vs. 58.7%; (53.3%) vs. 51.5%, respectively,P>0.05). In addition, fertilized embryos and pathenogenetic embryos have the same development speed. Parthenogenetic embryos could be used to detect the culture system in vitro as well as fertilized embryos.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2004年第6期612-614,共3页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(39830280)
国家"863"高技术研究发展计划(2001AA21380)资助
