摘要
对突触素(synaptophysin)、神经肽Y(NPY)在链尿佐菌素诱导的糖尿病模型大鼠额叶皮质和海马组织细胞中的表达进行研究,并利用UTHSCSA Image Tools 3.0进行图象分析,同时对其与学习记忆的关系进行探讨。选取成年Sprague-Dawley雄性大鼠20只,体重200—300g,随机分为两组。实验组用链尿佐菌素诱导产生糖尿病模型,并以血糖测定和尿糖水平测定进行筛选,另一组为空白对照组。继续饲养4周后,各组大鼠先进行Y型迷宫测试其学习记忆能力,然后取出脑组织,制做连续冰冻切片,对大脑额叶皮质和海马组织进行突触素、神经肽Y酶标免疫组织化学染色,观察这些蛋白在糖尿病大鼠和正常大鼠脑中表达的差并。结果发现糖尿病大鼠在Y型迷宫测试中,错误次数明显增多,糖尿病大鼠额叶皮质和海马神经元数目较正常对照组明显减少,神经细胞内突触素和神经肽Y的表达均较正常对照组明显下降。我们的研究显示突触素和神经肽Y在糖尿病大鼠大脑额叶皮质和海马组织内表达的减少可能与糖尿病组神经细胞突触数目及突触的可塑性下降、学习和记忆能力障碍有关。这可能是造成糖尿病性痴呆的一个因素。
The synaptophysin(Syn)and neuropeptide Y (NPY) expressions in brain tissue of diabetic model rats were investigated.20 adult Sprague-Dawley male rats were randomly divided into two groups.10 rats were injected with streptozotocin to induce diabetes,which was identified by blood & urinary sugar level;and the others were injected with sodium citrate buffer as the control.After 4 weeks diabetes appearance,the learning and memory ability of each rat in both group were tested by Y-Maze.Afterward freeze sections from the frontal cortex and hippocampus were made and stained with Nissl and Syn,NPY Immunohistochemistry staining.It was observed that diabetic rats showed more errors in Y-Maze test than those control rats.Both the number of neurons and the light densities of Syn and NPY immunostaining reaction in the frontal cortex and hippocampus of brain tissue sections significantly decreased in the diabetic model rats.It is sug- gested that the decrease in neuron number and Syn,NPY expression in frontal cortex and hip- pocampus may be one of the factors leading to diabetic dementia.
出处
《实验生物学报》
CSCD
北大核心
2004年第6期449-455,共7页
Acta Biologiae Experimentalis Sinica
基金
浙江省教育厅资助项目(No.20030309)。
