摘要
目的 采用高效液相色谱法同时测定三七中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量。方法 采用luna C18(2)分析柱;以(A)乙腈-(B)0.05％磷酸二元线性梯度洗脱:0-8 min(20％A:80％B),8-20 min[(20％A-40％A):(80％B-60％B)],20-30 min[(40％A-20％A):(60％B-80％B)],检测波长203 nm。结果 测得三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的回收率分别是(97.7±1.6)％、 (98.6±1.1)％、(99.2±0.8)％。线性范围分别是:三七皂苷R112-150μg·mL-1(r=0.9990)、人参皂苷Rg1 40500μg·mL-1(r＝0.999 4)、人参皂苷Rb1 44-550 μg·mL-1(r=0.999 7)。结论 采用高效液相色谱梯度洗脱法能将多种皂苷很好地分离检测,方法准确可靠,重复性好,结果稳定。
OBJECTIVE To determine notoginsenoside R1 , ginsenoside rg1 and Rb1 in radix notoginseng by HPLC linear gradient elution method. METHODS The luna C18 (2) column was used with a mobile phase of (A) acetoni trile- (B) 0.05% phosphoric acid gradient elution: 0 - 8 min (20% A ' 80% B), 8 - 20 min [(20%A-40%A) : (80%B-60%B)], and 20-30 min [(40%A-20%A) : (60%B-80%B)]. The detec tive wavelength was 203 nm. RESULTS The linear range of notoginsenoside R1 , ginsenoside Rg1 and Rb1 was 12 -150 μg · mL-1 (r=0. 9990), 40 - 500 μg · mL-1 (r=0. 9994), and 44-550 μg · mL-1 (r=0. 999 7), respec tively. The average recovery rate was (97. 7 ± 1. 6)%, (98. 6 ± 1. 1)% and (99. 2 ± 0. 8)% individually. CONCLU SIONS The method is rapid, accurate, sensitive and reliable, which can be used to control the quality of radix notog inseng products.
出处
《中南药学》
CAS
2004年第6期344-346,共3页
Central South Pharmacy
