摘要
以黄早四×Mo17自交形成的191个F2单株为作图群体,利用240对SSR引物和280对AFLP选扩引物,在亲本黄早四和Mo17之间进行了多态性检测,筛选出91对SSR引物和20对AFLP选扩引物用于F2群体分析。利用上述引物组合共检测到248个多态性标记位点,其中的218个标记构建了玉米分子连锁图谱,该图谱覆盖基因组全长2015.5cM,标记间平均间距9.69cM。
191 F_2 individuals derived from the single cross of inbred lines Huangzao 4 and Mo17 were used as mapping population in maize.240 SSR primer-pairs and 280 selective primer-pairs were used to detect the polymorphisms between parents Huangzao 4 and Mo17.91 SSR primer-pairs and 20 AFLP selective primer-pairs were selected to analyze F_2 population.248 markers were detected by the primer-pairs mentioned above.Only 218 markers were used to construct the molecular genetic linkage map,which span maize genome about 2015.5cM,with average distance of 9.69 cM between markers.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2004年第12期28-32,37,共6页
Journal of Northwest A&F University(Natural Science Edition)
