摘要
采用数理统计法对冬虫夏草菌丝液体培养进行了研究。通过对比试验结果得出:葡萄糖和玉米粉是适宜的复合碳源;酵母膏和黄豆粉是适宜的复合氮源。正交设计试验了培养基的成分及其较优浓度,再以中心组合试验对培养基成分进一步优化组合,并以回归方程对试验结果拟合。得出最佳液体发酵培养基配方(%):葡萄糖1.23,玉米粉1.5,黄豆粉2.0,酵母膏0.57,麦芽汁1.0,其碳氮比在4.8:1。
The liquid culture of Cordyceps Sinensis mycelium was studied by statistical methods. The contrast experiment results showed that, glucose and corn powder were the adequate multi-carbon sources; yeast extract and soya flour were the adequate multi-nitrogen soures. Nutyitional ingredients and their adequate combinationins were investigated by orthogonal devices. The optimum ratio of the ingredients was studied by the central combination design. The result was proved by regression equation in theory and the optimum nutyitional ingredients formalation percentages were(%): glucose 1.23, corn power 1.5, soya flour 2.0, yeast extract 0.57 and malt extract 1.0 formalafion. The ratio of C/N was 4.8:1.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第12期108-111,共4页
Food Science
关键词
数理统计法
优化
冬虫夏草
菌丝体
培养基
statiatical methods
optimize
Cordyceps Sinensis
mycelium
medium
