摘要
目的 研究低剂量环磷酰胺(CTX)对肺癌肿瘤血管生成的影响,观察其抑瘤效果及对生存期影响。方法 以荷Lewis肺癌的C5 7/BL6小鼠为模型,分别给予低剂量CTX、最大耐受剂量(MTD)CTX灌胃,免疫组化染色测定肿瘤微血管密度(MVD)、血管内皮生长因子(VEGF)和核增殖抗原表达基因(Ki- 6 7) ,观察小鼠生存期。结果 低剂量CTX治疗组小鼠肿瘤MVD值较对照组和MTDCTX治疗组均降低,同时低剂量CTX组VEGF及Ki- 6 7表达也降低(P <0 .0 5 ) ;低剂量CTX治疗组小鼠生存期较MTDCTX治疗组延长(P <0 .0 5 )。结论 持续低剂量CTX给药方式可抑制肺癌肿瘤微血管生成,具有良好抑瘤效果,动物生存期延长。
Objective To evaluate the efficacy of low-dose cyclophosphamide (CTX) for the antiangiogenic effect on lewis lung carcinoma, investigate its antitumor effect and animal survival.Methods C57/BL6 mice bearing lewis lung carcinoma were randomized into several groups, each received low-dose CTX?maximum tolerate dose (MTD) CTX therapy respectively. At the end of experiment, tumors were resected for immunohistochemical staining. Tumor microvascular density (MVD)?vascular endothelial growth factor (VEGF) and Ki-67 labeling index (Ki-67 LI) were detected. Survival of mice were monitored in each group.Results MVD of tumors were much lower in mice received continous low-dose CTX therapy; than those in MTD CTX group and control group. In low-dose CTX therapeutic group VEGF expression and Ki-67 LI were also decreased and the survival of mice was prolonged.Conclusion The continous low-dose regimen of CTX increases the efficacy of targeting the tumor microvasculature, and produces therapeutic activity with prolonged survival.
出处
《实用肿瘤学杂志》
CAS
2004年第6期411-413,共3页
Practical Oncology Journal
基金
黑龙江省科技攻关项目 (GC0 3C60 4-2 )
