摘要
目的 :探讨珊瑚转化羟基磷灰石 (CHA)作为骨组织工程支架材料的可行性 ,寻找最佳支架材料 ,为组织工程研究开辟新的途径。方法 :取 4周龄兔骨髓 ,分离骨髓基质细胞 ,体外培养 ,经诱导分化为成骨细胞 ,胰酶消化后离心收集细胞 ,接种至高温灭菌的CHA材料 ,无菌条件下植入裸鼠皮下组织中 ;对照组单纯植入CHA。分别于 6、8周取材 ,行大体观察、X线摄影及组织学染色 ,观察新骨形成情况。结果 :6周时X线片有高密度阻射影像 ,HE染色可见有新骨形成 ;8周时X线阻射影像密度更高 ,HE染色可见大量新骨形成并相互连接成骨梁样结构 ,骨细胞位于陷窝中。结论 :CHA可以作为骨组织工程的支架材料 。
Objective:To investigate the feasibility of using coralline hydroxyapatite (CHA) as scaffolds in bone tissue engineering and to find out the best scaffold material.Methods:Marrow stromal cells of four-month old New Zealand rabbits were isolated and culutured in vitro.Then the cells were induced to differentiate into osteoblasts.Afterward,the cells were harvested through pancreatin digestion and inoculated with CHA material undergone autoclaving.The osteoblasts/CHA complexs were transplanted subcutaneously into nude mice.CHA alone was transplanted as control.Osteanagenesis was evaluated at 6 and 8 weeks after the transplantation by gross observation,histology and roentgenography.Results:On roentgenograms,high density of imaging was present six week after the transplantation;new bone was found on HE staining sections;at the eighth week of the transplantation,much higher density of imaging was detected;on HE staining sections,a great quantity of osteanagenesis was noticed to form bone trabecula-like structure with osteocytes in osseous lacuna.Conclusions:CHA could be used as a scaffold material for bone tissue engineering.And it might open a vast range of prospects for its application to clinical practice.
出处
《华北国防医药》
2004年第6期383-385,F003,共4页
Medical Journal of Beijing Military Region
基金
国家"973"子课题口腔颌面骨组织工程 (G19990 5 43 0 8)
国家自然科学基金资助项目 ( 3 9779762 )
