摘要
目的 探讨严重腹腔感染时肠上皮细胞增殖细胞核抗原(PCNA)的表达及其意义。方法 SD大鼠60只,随机分成感染组和对照组,每组30只。感染组采用盲肠结扎加穿孔术制作严重腹腔感染模型,对照组仅行单纯剖腹手术。分别于术后1、2和4 d每组剖杀10只,取空肠、回肠标本,在光镜和电镜下观察病理组织学变化,采用免疫组化法检测PCNA指数。结果 对照组回肠上皮细胞微绒毛排列整齐,大小较一致;感染组肠上皮细胞微绒毛稀疏,排列不整齐,术后4 d尤为明显。与对照组比较,感染组术后1 d、2 d PCNA指数均明显增高(P均<0.01);组内不同时间比较,术后1 d最高,以后逐渐下降(P均<0.01)。结论 严重腹腔感染早期肠上皮细胞PCNA指数增高,提示PCNA表达与严重腹腔感染时肠黏膜损伤修复密切相关。
Objective To explore the expression and significance of proliferating cell nuclear antigen (PCNA) in intestinal epithelial cells during critical intraperitioneal infection. Methods A total of 60 SD rats were divided randomly to two groups: control group and infection group (30 rats in each group). Cecal ligation and puncture (CLP) was done in the infection group, and laparotomy only in the control group. Immunohistochemical technique was applied for assaying PCNA index of the jejunum and ileum samples on postoperative day 1, day 2 and day 4. Results The inflammatory reaction of small intestine mucous membrane was severer in the infection group than the control group. Compared with the control group, the PCNA indexes on day 1 and day 2 in the infection group were higher significantly (both P<0.01). The highest PCNA index was seen on postoperative day 1 in the infection group, then it lowered gradually (both P<0.01). Conclusion The PCNA index of intestinal epithelial cells is elevated during the early period of critical intraperitoneal infection. The findings indicate that PCNA index is related with the injury and repair of intestinal mucosa during critical intraperitoneal infection.
出处
《中国危重病急救医学》
CAS
CSCD
2004年第12期738-739,F006,共3页
Chinese Critical Care Medicine
基金
广东省自然科学基金资助项目(032901)
广东省重点科技攻关项目(2002C31004)
关键词
腹腔感染
增殖细胞核抗原
肠上皮细胞
<Keyword>intraperitoneal infection
proliferating cell nuclear antigen
intestinal epithelial cells
