摘要
以天然牛骨为原料,经过加工处理,分别在1 120和1 250℃烧结得到两组牛煅烧骨样品。测定了材料的物理性能,讨论了烧结温度对材料孔隙率及其结构等的影响。将两组煅烧骨样品分别与成骨前体细胞在体外复合培养,通过对细胞生长和分化情况的观察和测定,对两组多孔材料与成骨细胞的亲和性作出了评价。结果表明牛煅烧骨的主要成分为羟基磷灰石,保留了天然骨的网状孔隙结构,成骨细胞在两组材料上均能正常生长和分化,而且在1 120℃烧结的样品具有更好的成骨细胞亲和性,说明牛煅烧骨是一种有发展前途和应用前景的骨修复材料和骨组织工程支架材料。
Hydroxyapatite derived from nature bone has the characteristics of good biocompatibilities, suitable surface chemistry and three dimension porous-network systems. Two groups of porous hydroxyapatite ceramics, sample 1 and sample 2, were prepared by sintering of bovine bone at 1 120 and 1 250℃, respectively. They were characterized by scanning electron microscopy (SEM), mercury intrusion porosimetry, wide angle X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FT-IR). Further, MTT assay was used to investigate the proliferation of mouse preosteoblastic cells cultured on the hydroxyapatite scaffolds, and the activity of alkaline phosphatase (ALP), a marker for osteoblast differentiation, was quantitatively detected. The cells attachment on the materials were observed by SEM.The porous materials of high purity consisted of a unique normal hydroxyapatite structure was confirmed by XRD and FT-IR measurements. When cells were cultured on the hydroxyapatite scaffolds, 1.0 cm × 1.0 cm × 0.2 cm size, no proliferation inhibition was found. Osteoblasts proliferation was significantly greater on sample 1 than on sample 2 after 1, 3, 7 and 10 days of culture. There was significant difference on ALP activity of osteoblasts cultured on sample 1 in comparison with sample 2 after 7, 14, 21 and 28 days. Compared to sample 2, sample 1 turned to be excellent scaffold materials. These results suggest that bovine hydroxyapatite is a promising material for bone repairing and provide further basis for the use of natural bones as the scaffold in bone tissue engineering.
出处
《生物物理学报》
CAS
CSCD
北大核心
2004年第6期483-488,共6页
Acta Biophysica Sinica
基金
国家"863计划"项目(2001AA620506)
国家科技部"973"重大基础研究项目(G1999064702)
关键词
羟基磷灰石
骨
结构特征
生物相容性
Hydroxyapatite
Bone
Characterization
Biocompatibility
