摘要
目的探讨黄芪对大鼠急性肾缺血再灌注损伤的部分保护作用及机制。方法实验大鼠分正常组,模型组(缺血再灌注组)和黄芪组。分别在缺血1h、再灌注1h及24h检测肾组织一氧化氮(NO)含量和超氧化物歧化酶(SOD)活性的变化。结果与正常组比较,模型组NO含量在再灌注1h显著下降(P<0.05)、24h显著升高(P<0.01);黄芪组NO含量在缺血1h和再灌注1h均显著下降(P<0.01),再灌注24h则差别无显著性意义(P>0.05)。模型组和黄芪组各时间点SOD活力均下降,显著低于正常组(P<0.05),但黄芪组均显著高于模型组(P<0.05)。结论急性肾缺血再灌注过程中熏黄芪可能通过减轻NO含量过低或过高的变化及减少氧自由基积聚造成的损伤而具有保护肾脏的作用。
Objective To investigate the protective effect and its mechanism of Astragalus on renal ischemia-reperfusion injury in rats. Methods The rats were divided into three groups, normal control, renal ischemia-reperfusion model and Astragalus groups. In Astragalus group animals were injected with Astragalus intraperitoneally daily for two weeks before onset of renal ischemia-reperfusion. Nitric oxide,NO, and superoxide dismutase,SOD, levels in renal tissues were measured 1h after renal ischemia, and 1h, 24h after reperfusion. Results Compared with the control group , NO level was significantly decreased (P<0.01)1 h after reperfusion and significantly increased(P<0.01)24h after reperfusion in ischemia-reperfusion group. Compared with the control group, NO was significantly decreased(P<0.01)1h after renal ischemia, and 1 h after reperfusion in Astragalus group, but there were no significant changes(P>0.05)24 h after reperfusion. In ischemia-reperfusion group and Astragalus group, the activity of SOD was significantly decreased ,P<0.05, compared with control group at the same time points. Whatever, in Astragalus group, the activity of SOD was significantly highter ,P<0.05, than that of ischemia-reperfusion group at the same time points. Conclusion Astragalus have protective effect on the renal tissue during the ischemia-reperfusion damage, which may be associated with the reducing NO production.
出处
《浙江医学》
CAS
2004年第12期900-902,共3页
Zhejiang Medical Journal
