摘要
A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best analysis conditions were obtained. In the buffer of 2.0 mmol/L HAc-3.0 mmol/L NaAc-10.0% CH 3OH at a separation voltage of 22 kV, Sinomenine in the sample could be separated and detected within 4 min. The linear mass concentration of Sinomenine is in the range of 1.0—36.0 μg/mL, the limit of detection reached 0.20 μg/mL. The method was used for the analysis of Sinomenine in Sinomenium acutum and tablets satisfactorily with a recovery of 96%—102%.
A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best analysis conditions were obtained. In the buffer of 2.0 mmol/L HAc-3.0 mmol/L NaAc-10.0% CH 3OH at a separation voltage of 22 kV, Sinomenine in the sample could be separated and detected within 4 min. The linear mass concentration of Sinomenine is in the range of 1.0—36.0 μg/mL, the limit of detection reached 0.20 μg/mL. The method was used for the analysis of Sinomenine in Sinomenium acutum and tablets satisfactorily with a recovery of 96%—102%.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第12期2256-2258,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 0 3 75 0 49)
广东省自然科学基金重点项目 (批准号 :0 2 180 8)资助
关键词
毛细管电泳
高频电导检测
非接触式电导检测
青藤碱
青风藤
Capillary electrophoresis
High frequency conductivity detection
Contactless conductivity detection
Sinomenine
Sinomenium acutum
