摘要
在压电石英晶体金电极表面形成流基丙酸（MPA）单分子膜，经NHS和EDC活化后固定血吸虫抗原（SjAg），研制成血吸虫抗体（SjAb）压电免疫传感器．研究测定了抗原价数及免疫反应平衡常数Kass，结果令人满意．
A Piezoelectric immunosensor has ben developed for the determination of SchistlksomaJaponicum antibody (S jAb). The crysha are AT - cut and have basic resoan heency of 9MHz. Immobilization of the SjAg to the crystal's surface was accomglished via a NHA - EDC - activatedcopolymer coating of 3 - Mercaptopropionic acid (MPA). The valent value of SjAg binding with SjAnand the affinity constan of immunoreaction in this experimental system were studied. The analfocal re-sults given by this approarh trere satisfactory- Afer washing tvith Piranha solution, a crystal can be re-used two hundred times without detectable loss of sensitivity
出处
《怀化师专学报》
1998年第5期51-54,共4页
Journal of Huaihua Teachers College
关键词
抗原
免疫反应
日本血吸虫
NHS
抗体
固定
巯基
平衡常数
单分子膜
金电极
Piezoelectric immunosensor
Schistoksoma Japonicum antigen (SjAg )
Schistoksoma Japonicum antibody (SjAb)
Metcaptopropionic acid (MPA)
valent value
affinity constant
