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Clean SEA-TROSY Experiments to Map Solvent Exposed Amides in Large Proteins 被引量:1

Clean SEA-TROSY Experiments to Map Solvent Exposed Amides in Large Proteins
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摘要 It is well known that the SEA-TROSY experiment could alleviate some of the problems of resonance overlap in 15N/2H labeled proteins as it was designed to selectively map solvent exposed amide protons. However, SEA- TROSY spectra may be contaminated with exchange-relayed NOE contributions from fast exchanged hydroxyl or amine protons and contributions from longitudinal relaxation. Also, perdeuteration of the protein sample is a pre-requisite for this experiment. In this communication, a modified version, clean SEA-TROSY, was proposed to eliminate these artifacts and to allow the experiment to be applied to protonated or partially deuterated proteins and protein complexes. It is well known that the SEA-TROSY experiment could alleviate some of the problems of resonance overlap in 15N/2H labeled proteins as it was designed to selectively map solvent exposed amide protons. However, SEA- TROSY spectra may be contaminated with exchange-relayed NOE contributions from fast exchanged hydroxyl or amine protons and contributions from longitudinal relaxation. Also, perdeuteration of the protein sample is a pre-requisite for this experiment. In this communication, a modified version, clean SEA-TROSY, was proposed to eliminate these artifacts and to allow the experiment to be applied to protonated or partially deuterated proteins and protein complexes.
作者 林东海
出处 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2004年第12期1395-1398,共4页 中国化学(英文版)
基金 Project supported by the grants from One Hundred Talent Program of the Chinese Academy of Sciences and Shanghai Commission of Science and Technology (No. 03JC14081).
关键词 PROTEINS solvent accessibility hydrogen exchange TROSY NMR proteins, solvent accessibility, hydrogen exchange, TROSY, NMR
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