摘要
对太行菊生长期间茎顶芽和带腋芽的茎段进行组培试验。结果表明,对外殖体应用0.1%的HgCl2进行表面灭菌,5min处理茎顶芽和8min处理茎段的死亡率和感染率较低。外殖体组织分化有直接生成愈伤组织、小植株体,愈伤组织和不定芽同时生成三种形式。
The tissues cultures on stem apex bud and stem section with axillary bud of Taihang Chrysanthemum were studied. The results showed that the explants of Chrysanthemum treated by 0.1%HgCl2 in 5min (stem apex buds) and in 8min (steam section) could obtained low rate of dead and infection. The tissue disintegration of the explant could have the three forms that were, callus and plantlet growing directly and callus and adventitious buds growing at the same time. Tube Seedling is very good in take root.
出处
《中国农学通报》
CSCD
2004年第6期29-31,共3页
Chinese Agricultural Science Bulletin
基金
河南省科技攻关项目"太行山区野生花卉资源调查与产业化生产技术研究"
项目编号为991050038
关键词
太行菊
组织培养
外殖体
培养基
组织分化
Taihang Chrysanthemum, Tissueculture, Tissue disintegration
