摘要
自从Moore等提出色谱法定量分析氨基酸以来,传统的离子交换色谱及柱后茚三酮衍生检测方法始终占着主导地位。三十多年来,人们对该法及仪器做了大量的研究与改进工作,使分析的灵敏度、速度以及自动化程度有很大的进步,而且以此为基础,建立了食品、饲料等有关氨基酸分析的一系列国际和国家标准。然而,随着氨基酸分析应用领域的扩展,氨基酸测定仪用途单一、结构复杂、价格昂贵等缺点日益突出。自七十年代以来。
Reversed-phase high performance liquid chroma -tography has become a powerful method for assaying amino acids in biological material, food and feed. Four pre-column derivatization methods with O-phthal-dialdehyde ( OPA ), 9-fluotepylmethyl chloroformate (FMOC),phenylisothiocyanate(PITC) and 1-dimethyl aminonaphthalene-5-sulphonyl chloride ( dansyl-Cl ), were compared and evaluated.Special attention is paied to the comparison of accuracy, sensitivity, reproducibility and resolution with ion-exchange chrorratography method. In this paper we also give some typically chromatogram and summarize characterises of each methods.
出处
《色谱》
CAS
CSCD
北大核心
1993年第3期140-143,182,共5页
Chinese Journal of Chromatography
