摘要
为研究烟草尘能否引起以肺纤维化为主的尘肺病,将150只Wistar大鼠分为烟草组、正常组、石英对照组3组,分别注入50mg烟草尘、无菌生理盐水和50mg石英尘,染尘后分别于3,6,9及12月将动物处死,用比色、酶联免疫等方法测定各组大鼠的全肺干重、全肺胶原及Ⅰ型胶原含量,并用电镜观察了病理改变。结果:烟草组3项指标均高于正常组而低于石英组,且随实验时间的延长而增高;病理改变以轻度间质纤维化为主,伴有灶周和小叶中心性肺气肿,且随实验时间延长而逐渐加重。提示纯烟草尘能导致轻度的以肺间质纤维化为主的尘肺病。
The changes of dry weight,content of collagen and type 1
collagen of whole lung and pathologic morphology in normal rats and rats
injected into lungs with 50mg of pure tobacco and quartz dust respectively
intratracheally for 3,6,9 and 12 months were observed with the methods
of colorimetry,enzyme linked immunity and eletron microscope etc.The dry
weight,collagen contents,type 1 collagen contents of the whole lung in the
rats of pure tobacco groups were obviously higher than those in the normal
groups,but lower than those in the quartz groups,and increased with the
lapse of experimental time.The changes of pulmonary pathologic morphology
were mainly slighter interstitial fibrosis,and accompanied with perifocal and
centrilobular pulmonary emphysema,and gradually deteriorated with the
lapse of experimental time.These results indicated that the pure tobacco dust
may induce slighter pneumoconiosis and it were mainly interstitial fibrosis.So
that the occurrence of tobaccosis must be prevented
出处
《山东医科大学学报》
1993年第2期138-142,共5页
Acta Academiae Medicinae Shandong
关键词
尘肺
烟草
肺纤维化
比色法
Pneumoconiosis
Tobacco
Pulmonary fibrosis
Colorimetry
Enzyme-linked immunosorbent assay
Microscopy,electron
