摘要
目的 研究黄连解毒汤有效部位 (HLJDTAF)对小鼠的抗缺氧作用及对多发脑梗死大鼠能量代谢的影响。方法 通过小鼠常压耐缺氧、小鼠亚硝酸钠中毒和断头试验 ,观察黄连解毒汤有效部位连续灌胃给药 4d对缺氧的保护作用 ,同时观察该药对血细胞的影响 ;采用大鼠多发性脑梗死模型观察HLJDTAF对模型大鼠脑组织乳酸 (LA)、乳酸脱氢酶 (LDH)、Na+ K+ATP酶和Ca2 + Mg2 + ATP酶的影响。结果 HLJDTAF86 5 ,4 33,2 1 6mg·kg-1 可明显延长小鼠常压密闭状态下的存活时间 (与对照组比较P <0 .0 5 ) ;2 1 6mg·kg-1 可明显延长小鼠亚硝酸钠中毒缺氧存活时间 (与对照组比较P <0 .0 5 ) ;HLJDTAF86 5 ,4 33,2 1 6mg·kg-1 可明显延长正常小鼠断头张口喘气时间 (与对照组比较P <0 .0 5 ;P <0 .0 1 ) ,4 33mg·kg-1 可提高小鼠血液中血红蛋白含量 ,同时升高白细胞和淋巴细胞数量 (与对照组比较P <0 .0 5 ) ;HLJDTAF6 1 0 ,30 5mg·kg-1 组可显著降低多发性脑梗塞大鼠脑组织中LA含量 ,提高LDH活性 ,提高Na+ K+ ATP酶活性 ,1 5 3mg·kg-1 也可提高LDH与Na+ K+ ATP酶活性(与模型组比较P <0 .0 5 ;P <0 .0 1 )。结论 HLJDTAF可提高小鼠耐缺氧能力 ,同时改善多发脑梗死大鼠脑组织能量代谢障碍 ,这可能是其脑保护作用的机制?
OBJECTIVE To study the protection action of Huanglian Jiedutang active fraction(HLJDTAF) on the hypoxia mice and effect on energy metabolism of mutiple cerebral infarction(MCI) rats. METHOD By the ways of oxygen lack in normal atmosphere,NaNO_2 poisoning and decapitation of mice, the effect of HLJDTAF on the hypoxia and hemocyte were observed;MCI vehicle was used to observe the effect of HLJDTAF on lactic acid(LA), lactic dehydrogenase(LDH), Na+-K+ATPase and Ca 2+-Mg 2+ATPase in encephalon.RESULTS HLJDTAF 865, 433, 216mg·kg -1 could significantly prolong the living time of normal atmosphere,NaNO_2 poisoning and decapitation of mice(P<0.05;P<0.01 vs control), 433mg·kg -1 could increase the ferrohemoglobin, leucocyte and lymphocyte in mice (P<0.05 vs control). HLJDTAF 610,305mg·kg -1 could decrease the content of LA and increase the activity of LDH, Na+-K+ATPase and Ca 2+-Mg 2+ATPase in MCI rats encephalon.CONCLUSION HLJDTAF has certain protective action for hypoxia mice and ameliorate the energy metabolism in MCI rats encephalon, which may be the mechenism of its cerebral protective effect.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2004年第6期451-454,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
多发性脑梗塞
能量代谢
缺氧
mutiple cerebral infarction(MCI)
energy metabolism
hypoxia
