摘要
目的 研究以HPLC ELSD法测定虫草精口服液中黄芪甲苷的含量。方法 色谱柱 :Shim PackODS柱 (2 5 0mm× 4 .6mm) ;流动相 :乙腈 水 (36∶6 4 ) ;流速 :0 .8mL·min-1 ;进样量 :2 0 μL ;ELSD参数 :漂移管温度为 1 0 0℃ ,氮气流速 :2 .7mL·min-1 。结果 黄芪甲苷在 1 .6～ 8.0 μg之间线性关系良好 ,相关系数r=0 .9988;回收率为 98.89%,RSD =1 .3%。 结论 本法灵敏、简便 ,准确 ,可用于本制剂的测定和质量控制。
OBJECTIVE To establish a HPLC-ELSD method for determination of astragaloside Ⅳ in Chongcaojing solution.METHOD With a shim-pack ODS column (250mm×4.6mm),acetonitrile-water (36∶64) was used as the mobile phase,the temperature of drift tube was 100℃.RESULTS The linearity was obtained over the range of 1.6～8.0μg ( r=0.9988 ). The average recovery was 98.89% with RSD= 1.3%.CONCLUSION The result show that this method is sensitive, simple, specific and accurate for the determination of astragaloside Ⅳ in Chongcaojing solution.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2004年第6期485-487,共3页
Chinese Journal of Modern Applied Pharmacy
