摘要
背景与目的:关于survivin在结直肠癌中的表达及其与病理因素、细胞凋亡及血管生成的关系仍然不清楚。本研究旨在通过探讨survivin在结直肠癌中的表达对细胞凋亡的影响及其与血管生成的关系,以认识survivin对结直肠癌发展及预后的影响。方法:应用免疫组化技术检测survivin、血管内皮生长因子(vascular endothelial growth factor.VEGF)在91例结直肠癌组织中的蛋白表达,TUNEL法检测癌细胞凋亡指数(apoptosis index,AI)。结果:结直肠癌伴远处器官转移者的survivin蛋白表达(56%,14/25)高于未转移者(48.5%,32/66)(P<0.05),5年生存者survivin蛋白表达阳性率穴(n阳性患者平均生存期为91.3月熏阴性患者为116.4月,前者的5年生存率穴65.2%熏30/46雪显著低于survivin阴性者穴88.9%熏40/45雪穴P<0.01雪;survivin阳性患者的AI均数(0.74±0.19)%显著低于阴性患者(1.07±0.24)%穴P<0.01雪;survivin与VEGF蛋白表达显著相关穴Pearson系数:0.721雪。结论:survivin可抑制结直肠癌细胞凋亡和调节肿瘤血管生成。
BACKGROUND & OBJECTIVE: Expression of survivin, an apoptosis suppressor gene, in colorectal cancer(CRC), and its relationship with pathologic factors, cell apoptosis, and angiogenesis are unclear. This study aimed to investigate the effect of survivin on cell apoptosis, and its relation with angiogenesis, and to further explore the effects of survivin on development and prognosis of CRC. METHODS: Protein expression of survivin, and vascular endothelial growth factor (VEGF) in 91 specimens of CRC was detected by immunohistochemistry, apoptosis index (AI) of tumor cells was detected by TUNEL method. RESULTS: Positive rate of survivin in CRC with distant metastasis was 56.0% (14/25), significantly higher than that in CRC without metastasis (48.5%, 32/66) (P<0.05); and that in 5-year survival patients(42.9%,30/70) was lower than that in patients died within 5 years (76.2%,16/21) (P<0.01). The average survival time of patients with positive expression of survivin was 91.3 months, and that of patients with negative expression of survivin was 116.4 months; 5-year survival rate of the former (65.2%, 30/46) was significantly lower than that of the latter (88.9%, 40/45) (P<0.01). The average AI of patients with positive expression of survivin was lower than that of patients with negative expression of survivin [(0.74±0.19)% vs. (1.07±0.24)%, P<0.01]; the expression of survivin significantly correlated with that of VEGF (pearson: 0.721). CONCLUSION: Survivin may promote metastasis, and affect prognosis of CRC through inhibiting cell apoptosis, and regulating angiogenesis of CRC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2005年第1期116-120,共5页
Chinese Journal of Cancer
关键词
结直肠肿瘤
生存素
凋亡
VEGF
预后
Colorectal neoplasms
Survivin
Apoptosis
Vascular endothelial growth factor
Prognosis
