摘要
目的 观察中国人大肠癌K ras基因突变情况 ,并结合临床病理资料加以分析。方法 应用常规酚、氯仿法提取 48例散发性大肠癌组织及相应正常组织的DNA ,聚合酶链反应(PCR)扩增、单链构象多态性 (SSCP)电泳和DNA直接测序。结果 K ras基因突变率为 43 .8%(2 1/4 8) ,6种K ras基因突变类型被发现 ,包括 12密码子 (GGT→GTT、GGT→AGT、GGT→GAT、GGT→TGT)、13密码子 (GGC→GAC)和 61密码子 (CAA→CAT)。结论 中国人大肠癌K ras基因突变率为 43 .8% (2 1/4 8)。 12密码子 (GGT→GTT)是最常见的类型 ,61密码子是最少见的类型。中国人大肠癌K ras基因突变情况与欧美相似。大肠癌K ras基因突变与年龄、肿瘤部位、浸润深度、组织学类型、淋巴转移、远处转移、Dukes ,分期及 5年生存率无关。
Objective To detect K ras mutation in sporadic colorectal cancer in China and its correlation with parameters of clinical pathology.Methods DNA from 48 patients with sporadic colorectal carcinoma was extracted by standard phenol/chloroform method.K ras gene was amplified by using polymerase chain reaction (PCR) and analyzed by single strand conformation polymorphisms (SSCP) and DNA direct sequencing.Mutations in exon1,2,3 and 4 were analyzed.Results Six kinds of K ras gene were found,namely,codon 12 (GGT→GTT),codon 12 (GGT→AGT),codon 12 (GGT→GAT),codon 12 (GGT→TGT),codon 13 (GGC →GAC),and codon 61 (CAA→CAT).Conclusion The frequency of K ras gene mutation in sporadic colorectal carcinoma was 43.8% in China.Codon 12 (GGT→GTT) was the most common (33.3%, 7/21),while the codon 61(CAA →CAT) was the least (4.8%, 1/21).The spectrum of K ras gene mutation in China was similar to that of Europe and America.The frequency of K ras mutation is no correlated with the age,the position,the invasion depth,the histologic types,the lymph node metastasis,the Dukes' staging and the survival.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第1期65-67,共3页
Chinese Journal of Experimental Surgery
基金
广西十百千人才工程基金资助项目 (30 30 77)
