摘要
本文测定了接种马铃薯Y病毒脉坏死株系后烟草抗病转基因品系 960 8(转PVY -NIb的NC89)和非转基因NC89叶片组织透性和过氧化物酶活性 ,并对过氧化物酶同工酶带谱进行了分析。结果表明 ,抗病转基因 960 8和感病NC89叶片的组织透性与叶片内过氧化物酶活性的变化趋势一致 ,两者在接种后均呈增高趋势 ,但感病NC89的增高幅度明显高于抗病转基因 960 8;接种后 ,转基因 960 8和非转基因NC89的过氧化物同工酶均出现了一条新酶带 ,960 8的新酶带在接种后第 2d开始出现 ,而NC89的新酶带则在接种后第 6d开始出现 ,且明显强于 960 8。
The cell permeability, peroxidase (POD) activities and POD isozyme were systematically studied in the resistant transgenic NC89 tobacco line (9608) and nontransgenic susceptible NC89 leaves infected with patato virus Y vein necrosis strain (PVY N ). The changing trend of the cell permeability were consistent with that of POD activity both of them increased after PVY N inoculation, but the increase range in nontransgenic NC89 was greater than that in 9608. The number of bands of POD isozyme electropherogram didn't change in both of the 9608 and nontransgenic NC89 after infection by PVY N ,there was a new POD isozyme band either in 9608 or in NC89, but the new hand expressed at different time, it showed two days after inoculation in 9608 and showed six days after inoculation in nontransgenic NC89 leaves.
出处
《中国烟草学报》
EI
CAS
CSCD
2000年第3期23-26,共4页
Acta Tabacaria Sinica
基金
国家烟草专卖局科教司资助项目
