摘要
目的研究结直肠癌组织中血管内皮生长因子(VEGF)和血管形成因子-2(angiopoietin-2)及纤连蛋白(FN)的表达情况及其与结直肠癌肝转移的关系。方法获取结直肠癌标本60例,分别用SQRT-PCR和免疫组织化学染色法检测VEGF、血管形成因子-2、FN的mRNA转录和蛋白表达水平。结果结直肠癌组织中VEGF、血管形成因子-2和FN的mRNA转录和蛋白表达水平增高(P<0.05)。VEGF表达水平与肿瘤浸润深度、淋巴结和/或肝转移、Dukes分期有关(P<0.05)。血管形成因子-2则仅与癌组织的分化程度有关(P<0.05)。FN在细胞外基质(ECM)中的表达高于癌旁组织(P<0.05);而在基底膜中的表达低于癌旁组织(P<0.05);两者均与癌浸润深度和淋巴结转移有关(P<0.05)。而基底膜中FN的缺失同时与肝转移有关(P<0.05)。结论结直肠癌细胞产生的VEGF可能通过促进肿瘤新生血管的生成而促进血行转移和转移灶的形成,血管形成因子-2与VEGF共同促进肿瘤血管的生成,而FN在肿瘤组织基底膜中的缺失是肝转移的重要起始因素。
Objective To study the correlation of hepatic metastasis of colorectal cancer to vascular endothelical growth factor(VEGF),angiopoietin 2 and fibronectin(FN). Methods The transcription and expression of VEGF,angiopoietin 2 and FN were detected by semiquantitative reverse transcription polymerase chain reaction(SQRT PCR) and immunohistochemical staining in the specimens from sixty patients with colorectal cancer. Results The mRNA transcription and expression levels of VEGF,angiopoietin 2 and FN in colorectal cancer tissues were obviously higher than those in paratumor normal tissues(P< 0.05). The transcription and expression levels of VEGF were correlated with tumor invasion(P< 0.05),Dukesstage(P< 0.05),and lymph node and/or hepatic metastasis(P< 0.05). Angiopoietin expression and transcription levels were correlated with tumor differentiation. The expression of FN in extra cellular matrix(ECM) was significantly higher(P< 0.05),whereas ECM in basement membrane was significantly lower in cancer tissues than that in paratumor normal tissues(P< 0.05),which both were correlated with tumor invasion(P< 0.05),Dukesstage(P< 0.05),and lymph node metastasis(P< 0.05). Absence of FN protein in basement membrane was also correlated with hepatic metastasis. Conclusions Colorectal cancer cells can secrete VEGF and contribute to metastasis and proliferation of tumor by stimulating the growth of tumor vessel. Both of VEGF and angiopoietin 2 contribute to angiogenesis and the decrease of FN in basement membrane of cancer tissue is an important primary factor of hepatic metastasis.
出处
《中华胃肠外科杂志》
CAS
2005年第1期20-23,共4页
Chinese Journal of Gastrointestinal Surgery
