摘要
运用mRNA反转录差异显示技术可以了解不同细胞或同类细胞在不同发育阶段、不同生理状态下的基因表达状况 ,为研究生命活动过程提供重要信息。文章对差异显示反转录聚合酶链式反应技术的基本原理 ,优点与缺点 ,以及近年来对该技术涉及的RT PCR方法、引物设计、PCR反应参数、电泳及差异条带的显示方法以及杂交方式进行了论述和评价 ,并对其应用前景进行了简单分析。
DDRT-PCR technique is the available method very frequently to search and clone new genes.By using this technique it can comprehend the genic expression of different cells or the same cells in different periods.This technique provide important information for the investigation of all kinds of life activties. First this paper summarized the principled merits and shortage of DDRT-PCR.Secondly,it emphasized the improved techniques, which include the purification of mRNA, RT,the design of primer, parameters of PCR, electrophoresis, the marker of isotope, silver staining and the mode of hybridization and so on.Last,it introduced the development status of DDRT-PCR and analyzed the application of foreground.
出处
《动物医学进展》
CSCD
2005年第1期19-21,共3页
Progress In Veterinary Medicine
