摘要
目的:探讨趋化因子及其受体在变应性鼻炎发病机制中的作用。方法:采用基因芯片技术在芯片上 点阵30多个趋化因子及其受体的cDNA,对4例变应性鼻炎鼻黏膜及正常人鼻黏膜组织进行基因芯片检测,观 察趋化因子及其受体的表达情况。结果:在4例变应性鼻炎鼻黏膜组织标本中,存在eotaxin 1(CCL11),eotaxin 2(CCL24),MCP 3(CCL7),MCP 4(CCL13),RANTES(CCL5)等趋化因子和CCR2、CCR3、CCR4、CCR5等 趋化因子受体基因的差异表达,其中大多数趋化因子及受体倾向Th2优势。结论:变应性鼻炎存在Th免疫失 衡,趋化因子及其受体特别是Th2优势的趋化因子及受体在变应性鼻炎发病机制中发挥重要作用,这为变应性 鼻炎的诊治提供新的着眼点。
Objective:To analyze the role of chemokines and their receptors in the mechanism of allergic rhinitis(AR) by observing the expression of genes of chemokines and their receptors in nasal mucosa of AR through gene chip.Method:The total RNAs were isolated from nasal mucosa of AR and purified to mRNAs,then reversely transcribed to cDNAs and incorporated with fluorescent-labled CY5-dUPT for probes preparion.CY3-dUTP probes prepared with normal nasal mucosa of normal for control.The chip contains cDNAs of chemokines and their receptors were used to hybridized with probes then screened with computer to study the expression of genes based on different density of fluorescent.Result:The chemokines of CCL11(eotaxin-1),CCL24 ( eotaxin-2),CCL7(MCP-3),CCL13(MCP-4),RANTES(CCL5) and receptors of CCR2,CCR3,CCR4,CCR5 were differential expressed on four samples,and most of the chemokines and their receptors has tendency regulation on T help 2(Th 2) lymphocytes and involved in the prodeeds such as inducement of allergic reaction,accumulation of inflammacytes and degranulation of sensitized cells.Conclusion:A disorder exists in T helper immune system with AR.The chemokines and their receptors that polarized with Th 2 lymphocytes perhaps play important roles in AR pathogenesis, and it represents a new approach to AR immunotherapy.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第2期52-54,共3页
Journal of Clinical Otorhinolaryngology
基金
国家自然科学基金资助项目(No:30271406)
上海市优秀学科带头人"百人计划"基金资助项目(No:98BR003)
