摘要
研究了糙皮侧耳液体菌种适宜的培养条件、培养周期与贮存期。结果表明,糙皮侧耳液体菌种适宜的培养基配方为蔗糖2%,玉米粉4%,麦麸3%,酵母粉0.3%,KH2PO40.25%,Mg SO4·7H2O0.1%,ZnSO40.05%,pH6.17。摇瓶培养装液量80mL/250mL,摇床转速150r/min,接种量10%,培养周期6d,菌种于3d内用完为宜。
The suitable culture conditions, the culture period and the preservation time of the liquid spawn of P. ostreatus were studied. The experiment results showed that the optimum formula for P. ostreatus liquid spawn medium was as follows: sucrose 2%, corn meal 4%, wheat bran 3%, yeast powder 0.3%, KH_2PO_4 0.25%, MgSO_4·7H_2O 0.1%, ZnSO_4 0.05%, and the optimum pH was 6.17. While the optimum medium capacity, the rotation rate, the spawning rate and the culture period were 80mL/250mL, 150r/min,10% and 6d resp.. And it would be better to use the spawn within 3 days.
出处
《食用菌学报》
2004年第4期44-50,共7页
Acta Edulis Fungi
基金
山东省济南市科技局科技攻关项目"食用菌菌种引进选育及研究"[济科合字(2001)第70-1号]的部分内容
关键词
糙皮侧耳
液体培养
菌种培养
培养条件
Pleurotus ostreatus
Liquid spawn
Culture condition
Culture period
Preservation time
