摘要
为研究硒对不同价态锰诱导的人神经母细胞瘤细胞 (SH SY5Y细胞 )脂质过氧化的影响 ,把SH SY5Y细胞分为空白对照组、二价锰损伤组、硒二价锰保护组、三价锰损伤组、硒三价锰保护组。空白组为对照 ,硒保护组用硒预处理 4h ,然后与锰损伤各组分别加入相应二价锰和三价锰 ,使二价锰和三价锰的终浓度为 0 .5mmol/L ,共同培养2 4h。测定细胞中的过氧化物歧化酶 (SOD)、丙二醛 (MDA)、谷胱甘肽过氧化氢酶 (GSH Px)的活性及计算抗氧化指数 (GSH Px/MDA)。结果显示 ,硒二价锰保护组、硒三价锰保护组的SOD、GSH Px酶的活性及抗氧化指数与相应的二价锰损伤组、三价锰损伤组相比均有不同程度的升高 (P <0 .0 5 ) ,而MDA的质量摩尔浓度则均有所下降。提示 :硒对不同价态锰诱导神经细胞产生的脂质过氧化有抑制作用。
The aim was to study the effects of selenium on manganese with different valances altering lipid peroxidation in neuron cell SH-SY5Y. The cells were divided into five groups: the control, manganese(Ⅱ)-treated group, manganese(Ⅲ)-treated group and groups of the latter two disposed by selenium. After 4 h, all of the groups except the control were exposed to manganese for 24 h. All of the groups were detected by biochemical analysis such as enzyme activity. The activities of SOD, GSH-Px and the rats of the selenium-pretreated groups are significantly higher than that of manganese(Ⅱ) and manganese(Ⅲ)-treated group(P< 0.05). The content of MDA was also increased. The results show that selenium could inhibit manganese with different valance altering lipid peroxidation.
出处
《首都医科大学学报》
CAS
2004年第4期421-423,共3页
Journal of Capital Medical University
基金
国家自然科学基金 (3 0 0 0 0 14 0 )
教育部科学技术研究重点项目 (0 0 13 2 )
北京市科技新星计划 (95 5 810 2 80 0 )
教育部"高等学校骨干教师资助计划"项目 (5 2 )
北京市教委基金资助项目
