摘要
目的观察高氟摄入对大鼠所生仔鼠颅骨及成骨细胞超微结构变化,探讨氟中毒与成骨细胞细胞周期和细胞凋亡的关系。方法常规饮食条件下,给雌鼠自由饮用含氟化钠0、50、100、150mgL蒸馏水2个月,随后与正常雄鼠交配,取仔鼠颅盖骨及成骨细胞做光镜和电镜检查;应用流式细胞术检测仔鼠成骨细胞凋亡的百分率和细胞周期。结果氟中毒仔鼠颅骨骨基质增生,胶原纤维堆积,排列紊乱,甚至断裂。成骨细胞异常增生。粗面内质网扩张,部分线粒体嵴断裂或消失,核出现凋亡特征。饮水中氟化钠浓度为150mgL,对成骨细胞OB的细胞周期和细胞凋亡均有影响,使S期细胞数增多,G2M期细胞减少;凋亡细胞百分率明显增多。结论过多的氟化物可以通过胎盘屏障直接作用于仔鼠颅骨成骨细胞,引起成骨细胞结构、细胞周期的改变,并可致细胞凋亡。
Objective To investigate the effect of high dose fluoride which ingested by female rats on morphologic change in rat offspring's bone and osteoblast, discuss the relation between the mechanism of fluorosis and cell cycle, cell apoptosis. Methods In stock diets condition, Wistar female rats drank distilled water containing 0、50、100、150mg/L NaF for 2 months, then they are mated with normal rats. The calvarium and osteoblast of offsprings were used to investigate the effects of fluoride on ultrastructure by LM and TEM. FCM was used to analysis cell cycle and apoptosis. Results The Electron microscope revealed the number of microvilli of osteoblasts were overall decreased in rat offsprings with fluorosis. There was mitochondrial swelling and dilation of the rough endoplasmic reticulum(RER). The matrix of calvarium was hyperplasia and collagen was accumulated and turbulenced. The nuclear manifested the apoptosis character. NaF at 150mg/L increased the osteoblast number of S phase with relative decrease of cell number of G_2/M phase, but did not change that in G_0/G_1 phase. The apoptosis percentage increased in this group. Conclusion Excessive fluoride can directly through the placental barrier, influence cell structure and cell cycle distribution of fluorosis rat offspring and render the cell cycle stagnant in S phase, induce apoptosis.
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第1期29-32,共4页
Journal of Hygiene Research
