摘要
目的观察膳食n6n3脂肪酸比值对淋巴细胞脂肪酸构成及细胞功能的影响。方法BALBc小鼠随机分为5组n6n3比值分别为1(A组)、75(B组)、15(C组)、30(D组)和正常对照组,其中实验组S∶M∶P模拟中国居民膳食脂肪酸摄入的S∶M∶P为1∶15∶1,正常对照组为AIN93G配方的1∶15∶37。基础饲料采用AIN93G配方,脂肪酸构成以食用油脂调配。饲养12周。测定小鼠T淋巴细胞功能,脾淋巴细胞脂肪酸构成、PGE2水平。结果n6n3比值接近1时,小鼠T淋巴细胞增殖活性、CD4+、CD8+T细胞比例、培养上清IL2、PGE2水平显著降低;淋巴细胞C18∶2、C20∶4、n6PUFA含量显著减少;C22∶6、C16∶1、C18∶1、总MUFA含量明显高于其他实验组。淋巴细胞C22∶6含量与淋巴细胞增殖活性显著负相关;C20∶5含量与CD4+T淋巴细胞比例、IL2水平显著负相关;C16∶1含量与CD4+、CD8+T淋巴细胞比例显著负相关。结论小鼠脾淋巴细胞的脂肪酸构成受膳食脂肪酸构成的影响;n6n3比值为1组与比值为30的膳食组相比较,小鼠T淋巴细胞增殖活性受到抑制。
Objective To study the effect of dietary n-6/n-3 PUFA ratio on spleen lymphocyte's function and fatty acid composition in mice.Methods Male BALb/c mice were divided into 5 groups: the dietary S∶m∶p was 1∶1.5∶1 n-6/n-3 PUFA ratio was 1,7.5,15,30 respectively, and in the control group, dietary S∶M∶P was 1∶1.5∶3.7,which was followed the AIN-93G formulation.All groups consumed purified diet based on the AIN-93G formulation.The different n-6/n-3 PUFA ratios in diets were varied by mixing of oils.After 12wks breeding,all mice were sacrificed,and the function and fatty acid composition of Lymphocyte,IL-2 and PGE_2 concentration were measured.Results When dietary n-6/n-3 PUFA ratio approximated 1,the lymphocyte proliferation,proportions of CD^4+ and CD^8+ T cell,IL-2 and PGE_2 concentration decreased significantly.The concentration of C_~18∶2 ,C_~20∶4 ,n-6 PUFA in lymphocyte decreased significantly;Meanwhile,the concentration of C_~22∶6 ,C_~16∶1 ,C_~18∶1 and total MUFA in lymphocyte significantly higher than other groups.The concentration of C_~22∶6 in lymphocytes were significantly negative correlated with the proliferation of spleen lymphocytes.The concentration of C_~20∶5 in lymphocyte was significantly negative correlated with the proportions of CD^4+ T cell and IL-2 level.The concentration of C_~16∶1 in lymphocyte was significantly negative correlated with the proportions of CD^4+ and CD^8+ T cell.Conclusion The fatty acid composition of diet affected that of lymphocyte in mice.Compared with the T lymphocyte function in diet with n-6/n-3 ratio approximated 30,the T lymphocyte function in mice was suppressed when dietary n-6/n-3 ratio approximated 1.
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第1期100-103,共4页
Journal of Hygiene Research
基金
广州市科技局研究项目(No.2002
LC0081)
