摘要
目的 探讨耳蜗螺旋神经节及耳蜗核线粒体DNA(mitochondrialDNA ,mtDNA)缺失在老年聋发病中的可能作用。方法 分别测试老年组及青年组大鼠听性脑干反应 (ABR)阈值 ,应用聚合酶链反应 (polymerasechainre action,PCR)技术对老年及青年大鼠耳蜗螺旋神经节及脑组织中耳蜗核mtDNA 4 834bp缺失片段进行检测。结果老年组及青年组大鼠ABR阈值分别为 71.0 0± 6 .75、39.83± 4 .4 5dBpeSPL。老年大鼠耳蜗螺旋神经节及脑组织中耳蜗核mtDNA483 4 缺失出现率分别为 6 6 .7%、10 0 % ;青年鼠耳蜗螺旋神经节及脑组织中耳蜗核均无此片段缺失。 结论 老年大鼠耳蜗螺旋神经节及脑组织中耳蜗核存在mtDNA片段缺失 ,并可能在老年聋发病中起重要作用。
Objective To investigate the possible association of mtDNA deletion in spiral ganglion of cochlear and cochlear nucleus of brain in young and elder rat with presbyacusis.Methods The mtDNA 4834 deletion in spiral ganglion of cochlear and cochlear nucleus of brain in rat was analyed by PCR.Results The deletion rate of mtDNA 4834 in rat with presbyacusis was 66.7% in spiral ganglion, 100% in cochlear nucleus of brain.While no deletion was detected in young rat.Conclusion Deletion in mtDNA of the spiral ganglion of cochlear and cochlear nucleus of brain was found in rat with presbyacusis, and it may play an important role in the pathogens of presbyacusis.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2005年第1期50-52,i001,共4页
Journal of Audiology and Speech Pathology
基金
国家自然科学基金资助项目 (编号 3 9670 781)
