摘要
目的 观察胆道梗阻后心肌损伤及乌司他丁 (UTI)的保护作用。方法 动态观测胆道梗阻大鼠的心肌组织丙二醛 (MDA )和超氧化物歧化酶 (SOD )、血清总胆红素 (TBIL )、碱性磷酸酶 (ALP)、肌酸激酶同工酶 (CK MB)、内毒素 (ET)、肿瘤坏死因子α(TNF α)含量 ;取左心室心肌行光电镜检查 ,并应用ABC免疫组化染色法 ,定位TNF α在心肌组织中的表达和分布。结果 胆道梗阻后 ,血清TBIL ,ALP ,CK MB ,ET ,TNF α的水平逐渐升高 ;心肌组织MDA含量逐渐升高 ,SOD含量逐渐减少 ,心肌TNFα表达升高 ;各UTI治疗组与同时相梗阻组比较 ,血清TBIL ,ALP ,CK MB ,ET ,TNF α水平及心肌组织MDA含量减少 ,SOD含量升高 ,心肌TNF α表达下降 ;光电镜下可见随胆道梗阻时间延长 ,心肌损害加重 ,各UTI治疗组与同时相梗阻组比较 ,心肌损害减轻。结论 UTI通过对抗自由基损伤、内毒素血症、TNF α的综合作用 ,对胆道梗阻所致心肌损伤有明显保护作用。
Objective To investigate the myocardial injury induced by bile duct obstruction and the protective effects ulinastatin(UTI). Methods Dynamic observation of of the levels of malondialdyhyde(MDA) and superoxide dismutase(SOD) of myocardial tissues, and the levels of serum TBil, alkaline phosphatase(ALP), MB isoenzyme of creatine kinase(CK MB), endotoxin (ET), and tumor necrosis factor(TNF α) in bile duct obstruction(BLD) rats and UIT treatment rats. The left ventricles of the rats were obtained for light and electronic microscopic observation . Immunohistochemical staining method of ABC used was to locate the expression and distribution of TNF α in myocardial tissues. Results After ligation of the common bile duct, serum TBIL, ALP, CK MB, ET, TNF α levels and myocardium MDA gradully increased, while SOD levels gradually decreased, and the expression of TNF α in myocardium increased. As compared with BDL group at the same phase, in UIT group, serum TBIL, ALP, CK MB, ET, TNFα levels and myocardium MDA in UTI treated groups decreased, while myocardium SOD increased, and the expression of TNF α in myocardium decreased.Myocardial injuries of bile duct obstruction were aggravated as time progressed, and there were less myocardial injuries in UTI treated groups than in BDL groups at the same stage as shown with light and electronic microscopic observation.Conclusions UTI can effectively protect the myocardium from ET,TNF α and free radical injury in bile duct obstucion rats.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第1期24-28,共5页
China Journal of General Surgery
