摘要
目的探讨黏膜佐剂霍乱毒素B 亚单位（CT-B ）在激发旋毛虫感染的黏膜免疫中的作用。方法24只NI H 小鼠随机分为4 组，每组6 只。CT-B 免疫组：每只小鼠于0 、7 、14 d 用含幼虫抗原100 μg 、C T-B 10 μg 的免疫原200 μl 经口灌服；正常对照组；免疫后感染组：免疫方法同CT-B 免疫组，末次免疫后第7 天，每只小鼠用200 条旋毛虫进行感染；单纯感染组：不免疫，与免疫后感染组同时进行感染。CT 蛳B 免疫组与正常对照组在免疫后第7 天、免疫后感染组与单纯感染组在感染后第7 天分别取血检测Ig A 、Ig G 1 、Ig G 2 b ，刮取肠黏液检测sIgA ，检测肠道成虫数，并收集每鼠雌虫，在体外检查其生殖力。结果与单纯感染组相比，CT-B 免疫后感染组肠道成虫数明显减少，雌虫生殖力明显降低，成虫与新生幼虫减虫率分别为88.2%和72.4%。CT-B 免疫组小鼠肠黏液sIg A 、血清Ig A 较对照组小鼠明显提高(0 .2 9 ±0 .04 vs 0.09 ±0.02，0.21 ±0.06 vs 0.08 ±0.01，P <0.001)；而两组Ig G 1 和Ig G 2 b 水平无显著性差异(P>0.05)。CT-B 免疫后感染组小鼠肠黏液sIg A 、血清Ig A 较单纯感染组小鼠相比有显著性差异(0 .5 5 ±0 .28 vs0.08 ±0.15，0.33 ±0.06 vs 0.10 ±0.10，P <0.001)。
objective To investigate the effect of CT-B on the mucosal immunity of mice infected with Trichinella spiralis(T. spiralis). Methods Twenty four NIH mice were randomly divided into four groups. Group1:immunized mice group was immunized orally with the mixture of 10μg CT-B and 100μg T. Sprialis muscle larval soluble antigen on day 0, 7 and 14 . Group 2: normal control group. Group 3: CT-B immunized and infected group ie, the mice were immunized as group 1, then challenged with 200 T. Spiralis infective muscle larvae on day 7 . Group 4: The only mice of infected group, were challenged stimutaneously with group 3. Group 3 and group 4 were sacrificed on the 7th day after their infection, intestinal adults worms, female worm fecundity and the level of sIgA and serum IgA were analyzed. Parallely the level sIgA and IgA in group 1 and group 2 were analyzing. Results Compared with INF group, CT-B+INF group showed a significant reduction in the number of intestinal adult worms and newborn larvae, the rate of reduction in adult worm and newborn larvae was 88.2% and 72.4% respectively. The level of sIgA and IgA was significantly higher in CT-B group than that in C group(0.29 ± 0.04 vs 0.09±0.02 and 0.21±0.06 vs 0.08±0.01 respectively, P < 0.001), but no significant difference was found in the level of IgG1 and IgG2b between the two groups(P > 0.05). Similarly, there was significant difference in the level of sIgA and serum IgA between CT-B+INF group and INF group(0.55 ± 0.28 vs 0.08 ± 0.15 and 0.33 ± 0.06 vs 0.10 ± 0.10,P < 0.001), no significant difference in the level of IgG1 and IgG2b between the two group(P > 0.05). Conclusions CT-B can induce not only mucosal immune responses by increasing the level of sIgA, but also systemic immune responses through enhancing the level of IgA, this suggests that CT-B can improve the effect of protective immunity to a subsequent challenge infection of T. Spiralis.
出处
《北京医学》
CAS
2005年第1期39-41,共3页
Beijing Medical Journal
