摘要
本研究采用了体外细胞培养技术 ,将人胚成骨细胞分别与不同生物陶瓷 (HA ,TCP ,FHA ,BGC)及复合生物陶瓷 (BMP ,TGF β1)共同培养。通过相差显微镜及扫描电镜观察材料表面及周围细胞形态及附着情况 ,分子生物学方法测定细胞增殖率 ,碱性磷酸酶 (ALP)、骨钙素 (OC)、白细胞介素 6 (IL 6 )及转化生长因子 β1 (TGF β1 )分泌水平 ,探讨其作用机理。结果发现复合生物陶瓷细胞增殖率 ,ALP、OC、IL 6及TGF β1 水平明显高于相应单一材料及空白对照。而不同的复合材料细胞增殖率及细胞基质蛋白分泌有所差异。FHA +BMP ,TCP +BMP及HA +BGC +TGF β1 显示了较高水平。不同材料培养不同时间对细胞生长及代谢影响有所不同 ,反映了成骨细胞培养方法评价人工骨替代材料成骨活性规律是切实可行的。
Human fetal osteoblast were used in vitro culture to evaluate the osteogensis of bioactlve ceramic materials composed of bone morphogenetic protein (BMP) and transforming growth factor β 1 (TGF-β 1) and singe ceramic materials. The phase contrast microscope and scanning electron microscope were used to evaluate the cell morphology and attachment. The content of cell proliferation, alkaline phosphatase (ALP), osteocalcin (OC), interleukin-6 (IL-6) and TGF-β 1 were calculated by molecular biological methods.The effect mechanism of bioactive ceramics on both of osteoblast and extracellulaf matrix was studied. The results showed that on the composition of ceramics(BMP, TGF-β 1),the proliferative rate, ALP, OC, IL-6 and TGF-β 1 were in higher level than those on relevant single materal and control group. The rate of cell pro1iferation and bone mattices formation on TCP+BMP, FHA+BMP, HA+BGC+TGF-β 1 were in higher level than other materices, and increased with time extension. The results indicate that methods of human fefal osteoblastic culture used to evaluate osteogenesis of artificial bone on reparation defect will be feasible.
出处
《北京生物医学工程》
2005年第1期17-23,63,共8页
Beijing Biomedical Engineering
基金
国家自然科学基金项目 (3 0 170 2 73 )资助
