摘要
与Gateway技术兼容的农杆菌双元载体系统已开始应用于植物功能基因组的研究 ,但应用这些载体系统的一个瓶颈问题 ,是如何简单、经济和高效地将PCR产物或其他来源的目的DNA片段构建到入门载体上获得入门克隆 .为此 ,将传统的TA克隆技术与Gateway重组克隆技术进行整合 ,构建了与Gateway技术兼容的两种TA克隆载体 ,用于在克隆PCR产物或其他来源的目的DNA片段的同时获得入门克隆 .利用兼容Gateway技术的TA克隆载体有效地解决了上述瓶颈问题 .
A Gateway-compatible Agrobacterium sp. binary vector system for high-throughput functional analysis of genes in plants has been produced, but a bottleneck problem using this system for fast and reliable DNA cloning is how to obtain entry clones for the PCR products or other DNA fragments simply, economically and efficiently. To address this problem, the traditional TA cloning and the Gateway recombinant cloning techniques are integrated, and two kinds of TA cloning vectors compatible with the Gateway cloning are constructed. The vectors can be used for cloning PCR products or other DNA fragments and at the same time making entry clones in a simple, economical and efficient way.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第10期951-954,共4页
Progress In Biochemistry and Biophysics
基金
国家重点基础研究发展规划项目 ( 973 ) (G19990 1170 0 )
国家自然科学基金资助项目 ( 3 0 3 0 0 0 2 5 )~~
