期刊文献+

Charcterization of Type Ⅱ Topoisomerase Gene Mutations in Clinical Isolates of Mycoplasma Hominis Resistant to Fluoroquinolones

Charcterization of Type Ⅱ Topoisomerase Gene Mutations in Clinical Isolates of Mycoplasma Hominis Resistant to Fluoroquinolones
下载PDF
导出
摘要 Objective: To analyze type II topoisomerase genes in clinical isolates of fluoroquinolone-resistant Mycoplasmahominis. Methods: Eight isolates of M.hominis cross resistant to 6fluoroquinolones were selected from 103 clinical strains ofM.hominis using a broth microdilution method. Type IItopoisomerase genes were amplified by PCR and directlysequenced. Nucleotide sequences were compared to sequencesfrom a susceptible strain (M.hominis PG2I). Results: MICs of resistant Mh isolates were 4- to 512-foldhigher than MICs from the susceptible reference strain.Sequence comparison revealed a C to T change at 113nt ingyrA QRDR led to the substitution of Ser83 by Leucine and noamino acid change in gyrB. A change of G to T at 134nt inparC QRDR led to the substitution of Ser80 by Isoleucine anda G to A change at 70nt in ParE QRDR led to the substitutionof Aspartic acid by Asparagine.Conclusion: These results suggest that a C to T change at113nt in gyrA, a G to T change at 134nt in parC and a G to Achange at 70nt i atrE are associated with fluoroquinolone resistance of M.hominis. Objective: To analyze type Ⅱ topoisomerase genes inclinical isolates of fluoroquinolone-resistant Mycoplasmahominis. Methods: Eight isolates of M.hominis cross resistant to 6fluoroquinolones were selected from 103 clinical strains ofM.hominis using a broth microdilution method. Type IItopoisomerase genes were amplified by PCR and directlysequenced. Nucleotide sequences were compared to sequencesfrom a susceptible strain (M.hominis PG2I). Results: MICs of resistant Mh isolates were 4- to 512-fold higher than MICs from the susceptible reference strain.Sequence comparison revealed a C to T change at 113nt ingyrA QRDR led to the substitution of Ser83 by Leucine and noamino acid change in gyrB. A change of G to T at 134nt inparC QRDR led to the substitution of Ser80 by Isoleucine anda G to A change at 70nt in parE QRDR led to the substitutionof Aspartic acid by Asparagine. Conclusion: These results suggest that a C to T change atll3nt in gyrA, a G to T change at 134nt in parC and a G to Achange at 70nt in patrE are associated with fluoroquinoloneresistance of M.hominis.
出处 《Chinese Journal of Sexually Transmitted Infections》 2002年第4期7-9,共3页 中华性传播感染杂志(英文版)
基金 Financially Supported by a Grant from the Education Committee of Hunan Province(No.OOAOO9)
关键词 Mycoplasma hominis GENE STRUCTURAL MUTATION FLUOROQUINOLONES 支原体感染 基因突变 临床研究 PCR 聚合酶链反应 核酸 基因序列 克隆 荧光镜检查 MICS 肺炎
  • 相关文献

参考文献1

二级参考文献2

共引文献102

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部