摘要
对50L内循环气升式发酵罐的混合、传质、气含率、液体循环等性能进行了系统的研究,优选出设备的最佳结构及得出氧的体积传质系数(k_L a)关联式。在此基础上,进行了谷氨酸的一次低糖发酵试验,得出适用于此设备的谷氨酸发酵最佳工艺。实验结果的最高产酸率为7.69%,转化率为58.8%,比使用同一菌种的用机械搅拌罐发酵的产酸率(6.0%)及转化率(44%)分别提高28.2%和33.6%。
The characteristics of 50 L air-lift loop reactor with internal circulation and its use for glutamic acid fermentation were investigated. The most suitable conditions with respect to the high mass transfer and efficient mixing were obtained. The results of fermentation show that the most suitable gas through-put for the glutamic acid(GA)fermentation is 1.3 volume of air per volume of fermentation liquor per minute(vvw) in the growth phase(early stage of fermentation), and 3.8 vvm in the GA production phase(late stage of fermentation). GA concentration at the end of the batch was 7.69% and the conversion rate to glucose was 58.8% (w/w), that respectively exhibited 28.2%, 33.6% increases compared to those obtained in a 20000L mechanically agitated fermentor.
出处
《生物工程学报》
CAS
CSCD
北大核心
1993年第2期170-174,共5页
Chinese Journal of Biotechnology
基金
国家七·五攻关项目子课题
关键词
气升式
发酵罐
谷氨酸
Air-lift
fermentor
glutamic acid
