摘要
本文采用0.83％碘乙酰胺(CIA)化学灭活剂和热处理灭活方法,对黑木耳单株原生质体灭活,观察了化学灭活与不同温度热灭活的灭活效果。并通过对灭活原生质体与未经灭活处理的原生质体融合后再生产物分析、证明,不论化学灭活还是热灭活处理,只要筛选好灭活条件,作为黑木耳种内原生质体融合遗传标记是可行的。
We used both 0 .83%(CIA)chemical inactivated and heat inactivated on the Auricularia auricula mono-strains protoplast. We observed the effect of the chemical inactivated and the effect of the heat inactivated at different temperature. We also analysed the regeration which was regerated after the inactivated pretoplast was tusion with infected protoplast. All these prove if we can choose the right condition no matter chemical inactivated or heat inactivated,it is possible for it to be used as the genetical marker in the Auricularia auricula protoplast fusion breeding.
出处
《生物技术》
CAS
CSCD
1993年第5期35-38,共4页
Biotechnology
关键词
黑木耳
灭活
原生质体
融合
育种
Auricularia auricula
Inactivated protoplast
Fusion
