摘要
利用石英表面沉积LB膜的紫外吸收特性与CD谱特性研究了磷脂单分子膜与SpA的相互作用,买验结果表明,单分子膜的疏密状态、蛋白质与膜表面的电荷特性及亚相的Ca^(2+)离子浓度均对该蛋白质与磷脂的相互作用有显著的影响。
The interaction of the phospholipids monolayer with SpA adsorbed from solution has been studied by ultraviolet and circular dichroism spectroscopies of films transferred from air-water interface of a Langmuir film balance. The experiment results revealed that the interaction was strongly influenced by the surface pressure of the monolayer. the charge states of the monolayer and the protein as well as the ionic strength of the subphase. The secondary structure of a portion of protein in the monolayer was altered by the penetration procedure from the subphase to the lipid monolayer.
出处
《生物物理学报》
CAS
CSCD
北大核心
1993年第4期541-546,共6页
Acta Biophysica Sinica
关键词
蛋白A
磷脂单分子膜
构象
生物膜
SpA Phospholipid monolayer Electrostatic interaction Conformation Density of protein
