摘要
目的：研究二甲基亚砜（ＤＭＳＯ）对人肺癌细胞凋亡的诱导作用。方法：用不同浓度的ＤＭＳＯ处理体外培养的人肺癌细胞Ａ５４９，应用普通光镜、荧光显微镜、ＭＴＴ分析方法和流式细胞技术（ＦＣＭ）检测肺癌细胞凋亡的形态学变化、细胞存活率、凋亡百分率和细胞周期分布的变化。结果：ＤＭＳＯ诱导Ａ５４９细胞核ＤＮＡ凝缩和核片段化，最后形成凋亡小体；随着ＤＭＳＯ浓度的增加和处理时间的延长，细胞存活率明显下降，其ＩＣ５０为３．２％；４％的ＤＭＳＯ处理细胞 １２ｈ，凋亡率高达３３．０％；同时Ｇ０／１期细胞明显憎加，Ｓ期和Ｇ２／Ｍ期细胞明显下降。结论：ＤＭＳＯ可诱导人肺癌细胞凋亡，并使细胞受阻于Ｇ０／１期而进入凋亡程序。
Objective: In order to study the apoptosis inducing function of dimethyl sulfoxide (DMSO) in human lung cancer cells A549. Methods: Light-and fluorescence microscope, MTT assay and flow cytometry (FCM) were used to determine morphological changes, cell viability apoptosis rate and cell cycle distribution. Results: DMSO induced the chromatin condensation, nuclear fragmentation and the formation of apoptotic body at the concentrations. Cell viability decreased with raised concentrations and prolonged treatment of DMSO; the IC50 was 3.2%; apoptosis rate was 33. 0% when the cells Were treated with 4% DMSO for 12h, at the same time, the cells in G0/1 phase increased and the number of cells in S and G2iM phase decreased. Conclusion: DMSO induces apoptosis and blockes the cell cycle at G0/1 phase in human lung cancer cells A549.
出处
《中国现代普通外科进展》
CAS
2001年第3期145-147,共3页
Chinese Journal of Current Advances in General Surgery
基金
教育部高校骨干教师科研基金项目
关键词
肺肿瘤
凋亡
二甲基亚砜
Lung neoplasms
Apoptosis
Dimethyl sulfoxide
