摘要
目的 建立小鼠硬皮病动物模型。方法 将 0 .0 1～ 1mg博莱霉素皮下注射于 6周龄BALB/c雌性小鼠背部皮肤 ,第日 1次共注射 4周。取注射区皮肤行光镜和电镜观察 ,并用免疫组化方法检测TGFβ1、TGFβ2 和α SMA的表达。结果 0 .1mg/d以上博莱霉素剂量在 4周后皆可致小鼠背部皮肤硬化 ,表现为皮肤弹性降低、毛发脱落、表皮萎缩、真皮炎细胞浸润、毛囊减少或消失、纤维组织增生、大量肌成纤维细胞增生 ,真皮TGFβ1、TGFβ2 和α SMA表达增强。结论 博莱霉素连续皮下注射可导致小鼠皮肤硬化 ,符合硬皮病皮肤基本的组织病理学改变。
Objective To establish animal model for scleroderma.Methods Bleomycin was subcutaneously injected into the shaved back skin of female BALB/c mice aged 6 weeks at a dosage of 0.01mg-1mg per day for 4 weeks. The skin change was observed by light and transmission electron microscopy. The expression of TGFβ 1 and a-sma TGFβ 2,was investigated by immunohistochemical staining. Results More than 0.1mg/d of bleomycin could induce dermal sclerosis on mice back skin after 4 weeks. Sclerotic skin with reduced elasticity and hairloss exhibited atrophy of epidermis, inflammatory infiltration in dermis, reduction or disappearance of hair follicles, fibroproliferation with a large number of myofibroblasts, and increased expression of TGFβ 1,TGFβ 2 and a-sma. Conclusion A mouse model for scleroderma can be successfully established in BALB/c mice after 4-week injection of bleomycin.
出处
《重庆医学》
CAS
CSCD
2004年第12期1821-1823,1825,共4页
Chongqing medicine
基金
国家自然科学基金课题 (30 1 0 0 1 62 )
