摘要
利用集群分类法 bulkedsegregantanalysis,BSA 对与马铃薯青枯病 Ralstoniasolanacearum 抗性连锁的分子标记进行了分析.以马铃薯青枯病高抗性的原始栽培种Solanumphureja获得的二倍体群体为作图群体进行AFLP标记的初步筛选,另选一个与作图群体有较大亲缘关系和相近遗传背景的二倍体群体对所获标记进行验证.在标记鉴定过程中使用了共性AFLP标记 commonAFLPmarker 的方法.通过与已构建的连锁图谱的比较分析,获得了4个与马铃薯青枯病抗性相关的4个AFLP标记ATG/CTC307.0,ATG/CTC246.0,ATG/CTC191.0和AAC/CAC79.0,将其分别定位于染色体1和12上,可望应用于其它相关研究.
The bulked Segregant Analysis (BSA) was used to examine and evaluate the feasibility and the efficiency of the identification of markers linked to resistance to bacterial wilt (Ralstonia solanacearum) in potato. A highly resistant primitive cultivated species Solanum phureja was employed to generate a mapping population to perform the bulked segregant analysis for screening of AFLP markers linked with the resistance. Another population which had genetic similarity to the mapping population was used for testing of the markers achieved. A novel strategy named common AFLP marker was used to identify the genomic position of the molecular markers in the linkage map mapped before. Several informative primer combinations were employed in the detection and the common AFLP markers ATG/CTC 307.0, ATG/CTC (246.0), ATG/CTC 191.0 and AAC/CAC 79.0 were considered as markers that associated with the resistance and located on the relevant chromosome maps. The markers were located on chromosome 1 and 12 and may be used for other related studies.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第2期269-274,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
863计划项目 (2003AA207130)
