摘要
目的 初步探讨树舌多糖GF对小鼠HepA瘤基因组甲基化的影响。方法 提取基因组DNA ,采用限制性酶切片段长度多态性分析的方法进行甲基化检测。结果 HpaII酶切结果 :树舌多糖组可见 3个条带 ,猪苓对照组可见 3个条带 ,阴性对照组可见 4个条带 ,正常对照组可见 2个条带。MspI酶切结果 :树舌多糖组可见 6个条带 ,猪苓对照组可见 5个条带 ,阴性对照组可见 5个条带 ,正常对照组可见 6个条带。结论 小鼠HepA瘤基因组DNA是低甲基化的 ,树舌多糖GF有阻碍小鼠HepA瘤基因组DNA低甲基化发生的趋势 ,可能还具有抗 5mC的突变的作用。
Objective: To study the affect of GAPSGF on DNA methlylation in HepA tissue . Methed: Extracted DNA is investgated by RFLP. Results: The result of HpaII: GAPSGF Group have three bands, Positive Control Group have three bands, Control Group have four bands, Normal Control Group have two bands. The result of MspI: GAPSGF Group have five bands, Positive Control Group have five bands, Control Group have five bands, Normal Control Group have six bands. Conclution: Geneome DNA of HepA tisse in mice is low methlylation, GAPSGF have the trend of holding back low DNA methlylation, and can hold back the mutation of 5mC.
出处
《中国优生与遗传杂志》
2005年第1期51-52,共2页
Chinese Journal of Birth Health & Heredity
